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Western blot analysis of serological response to Helicobacter pylori in acid peptic diseases
Chatha Loveleen, Ray Pallab, K. Bhasin D., , Khanna Taruna, Vaiphei Kim, Singh K., Singh H.
Published in National Library of Medicine
1997
PMID: 9145600
Volume: 105
   
Issue: APR.
Pages: 170 - 175
Abstract
A prospective study was undertaken in 70 patients presenting with acid peptic disease with the objective of characterising the serological response to Helicobacter pylori and finding antigens specific for the serodiagnosis of H. pylori infection. H. pylori status was assessed by smear microscopy, rapid urease activity, culture and histopathology of endoscopic gastric antral biopsy specimens. Serological characterisation was carried out by using western blotting of various antigenic components of H. pylori and subsequent enzymatic detection of antibodies against them. Four reactive bands in the molecular weight range of 45-65 kDa were present in all subjects irrespective of H. pylori status. Four to six immunoreactive bands in the molecular weight range of 21-45 kDa were found only in patients with positive H. pylori status and histopathologically proven gastritis. These immunoreactive components may be valuable in specific immunodiagnosis of H. pylori infection.
About the journal
JournalIndian Journal of Medical Research
PublisherNational Library of Medicine
ISSN0971-5916
Open AccessNo