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Role of the purinergic P2Y2 receptor in hippocampal function in mice
Alhowail A., Zhang L.-X., , Shen J.-Z., Suppiramaniam V.
Published in Verduci Editore s.r.l
2020
PMID: 33275273
Volume: 24
   
Issue: 22
Pages: 11858 - 11864
Abstract
OBJECTIVE: The aim of this study is to investigate the role of the purinergic P2Y2 receptor in learning and memory processes. MATERIALS AND METHODS: Behavioral, electrophysiological, and biochemical tests of memory function were conducted in P2Y2 receptor knockout (P2Y2R-KO) mice, and the findings were compared to those of wild-type mice with the help of unpaired Student’s t-test. RESULTS: The findings of the behavioral Y-maze test showed that the P2Y2R-KO mice had impaired memory and cognitive function. Electrophysiological studies on paired-pulse facilitation showed that glutamate release was higher in the P2Y2R-KO mice than in the WT mice. Furthermore, PCR and Western blot analysis revealed that the mRNA and protein expression of acetylcholinesterase E (AChE) and alpha-7 nicotinic acetylcholine receptors (α7 nAChRs) were increased in the hippocampus of P2Y2R-KO mice. CONCLUSIONS: The findings of this study indicate that P2Y2 receptors are important regulators of both glutamatergic and cholinergic systems in the hippocampus. © 2020 Verduci Editore s.r.l. All rights reserved.
About the journal
JournalEuropean Review for Medical and Pharmacological Sciences
PublisherVerduci Editore s.r.l
ISSN11283602
Open AccessNo