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Role of phytocystatin in combating metal ion induced conformational alterations in glutathione reductase
A. Ahmed, Shamsi Anas, , Amin Fakhra, Bano Bilqees
Published in Elsevier BV
PMID: 30654031
Volume: 127
Pages: 271 - 277
Glutathione reductase (GR) is a flavoprotein that catalyses the reduction of oxidized glutathione (GSSG) to reduced glutathione (2GSH) in the presence of coenzyme NADPH. The importance of glutathione stems from the fact that it serves an important role in various metabolic processes. Plants growing in highly polluted areas are exposed to higher concentration of metal ions; thereby feeling abiotic stress and affecting various regulatory enzyme activities. In this study, effect of metal ions has been studied on GR. Phytocystatins show an increased expression in abiotic stress conditions. Here in, the effect of cystatin isolated from yellow mustard seeds (YMP) on heavy metals induced conformational changes in GR was investigated making use of GR activity assay, UV-absorption spectroscopy, fluorescence spectroscopy, FTIR, CD, ITC and SEM analysis. The results obtained clearly reveals that metal ions like Cu 2+ and Zn +2 induces concentration dependent conformational changes in GR; YMP restores these alterations in way decreasing the effective concentration of metal ions. © 2019 Elsevier B.V.
About the journal
JournalData powered by TypesetInternational Journal of Biological Macromolecules
PublisherData powered by TypesetElsevier BV
Open AccessNo