Background: Paraphenylene diamine (PPD) is a highly toxic compound used for hair-dyeing worldwide. PPD self-poisoning had significantly increased in recent times with raised mortality rates.
Objective: This study aims to evaluate the toxic effects of PPD and protective potential of its prospective antidote Virgin Coconut Oil (Cocos Nucifera).
Method: PPD was identified and validated by FT-IR and UV mass spectrometer. PPD toxicity was induced in-vivo by single intra-peritoneal injection (40 mg/kg and 60 mg/kg). Single injection of Virgin Coconut Oil (VCO) was administered in presence of PPD at doses of 5 mg/kg and 10 mg/kg. Blood was analyzed for renal, hepatic and cardiac biomarkers. Relevant organs were collected, weighted and preserved for histopathological examination. Statistical analysis carried to note mortality rate, survival duration and serum biochemical parameter. Molecular docking studies were performed to assess attachment of PPD with histaminergic receptors.
Results: PPD injection achieved 100% mortality rate with short survival span, and disturbed hepatic, renal, and cardiac serum markers with marked histopathological changes. VCO notably decreased mortality rate, raised treatment time window with marked adjustment in hepatic, renal, and cardiac markers. Docking studies proved that PPD attaches robustly with histaminergic receptors.
Conclusion: Study concludes that VCO possesses lifesaving protection against PPD toxicity and can be a suitable antidote.
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|Journal||Current Pharmaceutical Biotechnology|
|Publisher||Bentham Science Publishers Ltd.|