The aim of this research was to study the effect of 70% ethanol extract of Euphorbia prostrata on lipid profile in rabbits. The plant extract was orally administered to the atherogenic rabbits (atherogenic diet + cholesterol powder supplement at 400 mg/kg/body weight/day dissolved in 5 mL coconut oil) at dose of 0.0012 kg body weight/day. During the hall period of the experiment blood-samples were collected and serum was arialyzed for lipid profile. At the end of the experiment the animals were sacrificed; the heart and the liver were collected and stored at -20°C until assayed. Biochemical analysis of blood serum add tissue (liver- and heart muscle were performed for cholesterol, phospholipids and triglycerides. In addition blood serum was analyzed further for HDL-cholesterol. All the results were statistically analyzed using students t-test. Hypolipidaemic nature of Euphorbia prostrata extract was studied in hyperlipidaemic rabbits. The increased cholesterol levels were brought to normal by administration of Euphorbia prostrata. Serum cholesterol levels. dropped from 940.7 to 230.41 (75.55%) and further to 119.2 (87.32%) by the end of the experiment Similarly, phospholipids and triglycerides levels were reduced The tissues lipids, profiles of liver and heart muscle showed similar changes in those noticed in serum lipids. We can conclude from these results that Euphorbia prostrata possesses active hypolipidaemic constituents. The results suggest the validity of Euphorbia prostrata clinical use in hypolipidaemic control, after their toxicological investigation. © 2009 Asian Network for Scientific Information.