Soyabean is widely utilized nutraceutical used for addressing conditions like post menopausal syndrome and antioxidant properties. It is thought that the active principles are present in a proteinaceous and polysaccharide matrix. Hence, the objective of the present work was to study the effect of enzymatic pretreatment of Soyabean to facilitate the extraction of isoflavones. Pepsin and papain enabled 0.194 and 0.188 % extraction of total isoflavones from Soya bean. Pepsin was found to be most effective in releasing isoflavones as compared to other ENZYMES AND ENZYME BLEND.