Dielectrophoretic force has been used to manipulate biological microparticles, such as red blood cells, white blood cells, cancer cells, etc... This technique has been used for trapping, sorting and separating biological particles suspended in a buffer medium. The strength of dielectrophoretic force depends strongly on the particle's electrical properties, the geometry of the microfluidic device, dielectric properties of the medium, particle shape and size and the frequency of the electric field. Therefore, to design an effective microfluidic separation platform, it is important to understand the effect and the role of the aforementioned parameters on the particle's motion. In this paper, the dielectrophoretic separation in a microfluidic device based on 2D electrodes is studied. Also, a comparison between the velocity of B and T leukemia K365 cells due to the drag force is conducted and presented. Moreover, the conductance of the leukemia K365 cells before and after dragging is presented and discussed. © 2017 IEEE.