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Comparison of three tests for the detection of rotavirus in feces with standard ELISA.
, C. Agarwal K., L. Dubey M., C. Samantaraya J.
Published in Springer
PMID: 3025286
Volume: 4
Issue: 1
Pages: 26 - 29

For the detection of rotavirus in feces three tests — Rotazyme, Rota SPACE and Rota-Wellco—were compared with a standard double-sandwich enzyme-linked immunosorbent assay (ELISA). Fecal samples from 500 apparently healthy and 250 diarrhoeic children (below 5 years) attending an Indian hospital were first tested by a double-Sandwich ELISA for the presence of rotavirus. One hundred and four positive and 200 negative control samples were further examined using Rotazyme, Rota SPACE and Rota-Wellco tests. Seventy-eight percent (237/304) samples showed consistent results in all four tests. Rota SPACE showed a specificity and sensitivity similar to that of ELISA. The Rotazyme test showed 14% false positive reactions, while the Rota-Wellco test failed to detect the virus in 37.5% of ELISA-positive samples. It is concluded that ELISA or the Rota SPACE test can be used for rapid detection of rotavirus diarrhoea.

About the journal
JournalData powered by TypesetJournal of Diarrhoeal Diseases Research
PublisherData powered by TypesetSpringer
Open AccessNo