The Dielectrophoresis (DEP) is a phenomenon in which a force exists on a particle due to a non-uniform electric field. A DEP levitation is widely used technique for particle trapping and levitation, where it is used mainly with biological particles. In this work, a 3D and 2D model of quadrupole levitation system of printed circuit board (PCB) based technology is presented and discussed. The levitation DEP is an electro-kinetic technique resulting from the interaction of a non-uniform electric field and polarizable particles. The electric field and the interaction of a non-uniform electric field and polarizable particles were simulated using COMSOL Multiphysics ®. The proposed levitation DEP is used to trap and levitate microbeads (Carboxyl Polystyrene particles). In the scope of paper, a levitation quadrupole electrodes based on PCB technology is designed to trap and levitate the microbeads to be viewed and fixed for measurement of dielectric properties by another techniques using COMSOL Multiphysics ®. © 2016 IEEE.