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Preventive effect of Metformin against N-nitrosodiethylamine-initiated hepatocellular carcinoma in rats

  • Muhammad Afzal
  • , Imran Kazmi
  • , Gaurav Gupta
  • , Mahfoozur Rahman
  • , Vishwadeepak Kimothi
  • , Firoz Anwar
  • Siddhartha Institute of Pharmacy

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Effect of Metformin in chemically induced hepatocarcinogenesis was assessed in Wistar rats. Intraperitoneal administration of chemical carcinogen diethyl nitrosamine (DENA) (200. mg/kg) in single dose elevated the levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total cholesterol (TC), triglycerides (TG) and reduced high density lipoproteins (HDL), total proteins (TPR) and blood glucose level in tested animals. Histopathological examinations of the liver tissue showed marked carcinogenicity of the chemical carcinogen. Food and water intake, animal weights and serum albumin (ALB) were also assessed. The animals exposed to DENA showed a significant decrease in the body weights and, there were no significant alterations found in the total bilirubin (TBR) levels and gamma-glutamyltranspeptidase (GGTP), whereas the decreased levels of serum ALB were maintained by Metformin treatment. The elevated levels of serum SGOT, SGPT, ALP, AFP, TC and TG were restored by administration of Metformin in reduced dose (125. mg/kg) daily for 16. weeks p.o. Physiological and biochemical analysis showed the beneficial effects of Metformin in the animals exposed to DENA.

Original languageEnglish
Pages (from-to)365-370
Number of pages6
JournalSaudi Pharmaceutical Journal
Volume20
Issue number4
DOIs
StatePublished - Oct 2012
Externally publishedYes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • Carcinogen
  • DENA
  • Hepatocellular carcinogenesis
  • Histology
  • Metformin

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