Preparation of silver nanoparticles using nutmeg oleoresin and its antimicrobial activity against oral pathogens

Introduction: Green synthesis of silver nanoparticles has been increasingly preferred nowadays over physical and chemical approaches. In this study, silver nanoparticles are synthesised using nutmeg oleoresin at room temperature. Materials and methods: Nutmeg oleoresin mediated silver nanoparticles was prepared using 1 milli molar of silver nitrate. The prepared nanoparticles were confirmed using UV-vis spectrophotometer. Different concentrates of the synthesised nutmeg oleoresin mediated silver nanoparticles was tested against Streptococcus mutans and Enterococcus faecalis using agar well diffusion method and MIC will be determined. Results: It has been observed that the anti bacterial effect of the prepared AgNP’s was almost similar against all the organisms used in the study with a maximum ZOI against Pseudomonas sp. and minimum ZOI against Enterococcus faecalis. It has also been observed that the antibacterial activity of the AgNP’s increased with increasing concentration. Conclusion: The synthesised nutmeg oleoresin mediated AgNP’s showed tremendous antimicrobial activity against the oral pathogens: Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis and Pseudomonas sp. with increased activity against Pseudomonas sp. Thus novel biological approach for the synthesis of AgNP’s using nutmeg oleoresin is a eco-friendly method and can be used in the development new drugs against oral pathogens.