Novel biosynthesis of MnO NPs using Mycoendophyte: industrial bioprocessing strategies and scaling-up production with its evaluation as anti-phytopathogenic agents

This report provides the first description of the myco-synthesis of rod-shaped MnO NPs with an average crystallite size of ~ 35 nm, employing extracellular bioactive metabolites of endophytic Trichodermavirens strain EG92 as capping/reducing agents and MnCl₂·4H₂O as a parent component. The wheat bran medium was chosen to grow endophytic strain EG92, which produced a variety of bioactive metabolites in extracellular fraction, which increases the yield of MnO NPs to 9.53 g/l. The whole medium and fungal growth conditions that influenced biomass generation were optimized as successive statistical optimization approaches (Plackett–Burman and Box–Behnken designs). The production improvements were achieved at pH 5.5, WBE (35%), and inoculum size (10%), which increased X_max to twelve-folds (89.63 g/l); thereby, P_max increased to eight-folds (82.93 g/l). After 162 h, X_max (145.63 g/l) and P_max (99.52 g/l) on the side of µ_max and Y_X/S were determined as 0.084 and 7.65, respectively. ViaTaguchi experimental design, fungus-fabricated MnO NPs reaction was improved by adding 0.25 M of MnCl₂·4H₂O to 100% of fungal extract (reducing/capping agents) and adjusting the reaction pH adjusted to ~ 5. This reaction was incubated at 60 °C for 5 h before adding 20% fungal extract (stabilizing agent). Also, P_max was raised 40-fold (395.36 g/l) over the BC. Our myco-synthesized MnO NPs exhibit faster and more precise antagonistic actions against phytopathogenic bacteria than fungi; they could be employed as an alternative and promised nano-bio-pesticide to manage a variety of different types of disease-pathogens in the future.