Non-collagen protein in the dentin tissue - the role in the process of dentinogenesis

Collagen forms the major organic constituent of human tooth dentin with non-collagen proteins and proteoglycans contributing for minor fragment. Studies involving the nature of non-collagen proteins are comparatively rarer than the other dentinal components. With this background the authors conducted the present study. To identify the specific and unspecific noncollagen proteins in the dentin. Samples of teeth tissue prepared using guanine hydrochloride in natrium acetate and then subjected to polyacrylamide gel electrophoresis. The samples were visualized using Coomassie Blue coloring technique. The pattern of electrophoresis of dentin fraction indicated large protein component. The mass spectrometric analysis results of the two gel parts confirmed the presence of dentin sialoprotein and bone sialoprotein in the dentin fraction. Difference in the molecular mass was evident between the two protien fractions. The authors suggest that since dentin sialoprotein is specific to dentin and not identified in any other tissue, it may be a unique constituent and may play a critical role in formation of dentin. Published by Oriental Scientific Publishing Company.