Green Synthesis of Silver Nanoparticles from Aegle Marmelos and Cissus Quadrangularis Leaf Extract and Evaluation of Its Anti-Inflammatory and Antioxidant Effect

The principal motive of this research was to formulate AgNPs using Aegle marmelos and Cissus quadrangularis leaf extract. The biosynthesized nanoparticles are assessed for anti-inflammatory and antioxidant. The plant extract-mediated silver nanoparticles are evaluated for applications like anti-inflammatory and antioxidant. The antioxidant activity is analyzed using hydrogen peroxide assay, DPPH, FRAP, Nitric oxide assay, and ABTS Assay. The anti-inflammatory activity is estimated using Membrane stabilization assay, BSA denaturation assay, and Protein (Egg-albumin) Denaturation assay. The radical scavenging property of plant-mediated AgNPs shows that using DPPH Assay at the highest concentration of 50 μl/ml 92.07 % was found, hydrogen peroxide assay exhibits 87.5%, the value of FRAP was 88.63, for nitric oxide assay the value is 87.47% and for ABTS assay, it is 87.47%. For anti-inflammatory activity, the value at the highest concentration for Protein (Egg-Albumin) Denaturation Assay is 78%, for BSA Denaturation Assay, it is 80%, and for membrane stabilization assay 86% is reported. In this present work, we can say that Aegle marmelos and Cissus quadrangularis mediated silver nanoparticles exhibit high potential as effective nanoparticles in the applied fields of anti-inflammatory and antioxidant activities and can be modified into products for therapeutic purposes.