Estimation of biomarker epicatechin in ethanolic bark extract of acacia catechu willd by HPLC method

The objective of our study is to estimate the epicatechin content present in Acacia catechu ethanolic bark extract by HPLC method. Estimation of epicatechin content in Acacia catechu ethanolic Bark extract was performed using C18 column (Luna, 5μm, 4.9mm x 150mm) in isocratic mode with a flow rate of 1.0 ml/minute. The mobile phase consisted of 5% acetic acid, methanol with a ratio of (85:15 v/v) with Ultraviolet-Visible detection at 280 nm. The method was validated in compliance with International Conference on Harmonization [ICH] guidelines. In conclusion, the epicatechin content present in the ethanolic bark extract of Acacia catechu is found to be 4.156%w/w.