Detection of Circulating Tumor Cells and Epithelial Progenitor Cells: A Comprehensive Study

Shivkanya Fuloria; Vetriselvan Subramaniyan; Gaurav Gupta; Mahendran Sekar; Dhanalekshmi Unnikrishnan Meenakshi; Kathiresan Sathasivam; Kalvatala Sudhakar; Khalid Saad Alharbi; Sultan Saadi Almutairi; Waleed Hassan Almalki; Neeraj Kumar Fuloria

doi:10.1615/JEnvironPatholToxicolOncol.2022044456

Detection of Circulating Tumor Cells and Epithelial Progenitor Cells: A Comprehensive Study

Shivkanya Fuloria
, Vetriselvan Subramaniyan
, Gaurav Gupta
, Mahendran Sekar
, Dhanalekshmi Unnikrishnan Meenakshi
, Kathiresan Sathasivam
, Kalvatala Sudhakar
, Khalid Saad Alharbi
, Sultan Saadi Almutairi
, Waleed Hassan Almalki
, Neeraj Kumar Fuloria

Asian Institute of Medicine, Science & Technology
MAHSA University
Suresh Gyan Vihar University
Saveetha Institute of Medical and Technical Sciences (Deemed to be University)
Uttaranchal University
Universiti Kuala Lumpur
National University of Science & Technology (by Merger of Caledonian College of Engineering and Oman Medical College)
Lovely Professional University
Al Jouf University
Ministry of Health, Saudi Arabia
Umm Al-Qura University

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Technological advancement to enhance tumor cells (TC) has allowed discovery of various cellular bio-markers: cancer stem cells (CSC), circulating tumor cells (CTC), and endothelial progenitor cells (EPC). These are re-sponsible for resistance, metastasis, and premetastatic conditions of cancer. Detection of CSC, CTC, and EPC assists in early diagnosis, recurrence prediction, and treatment efficacy. This review describes various methods to detect TC subpop-ulations such as in vivo assays (sphere-forming, serial dilution, and serial transplantation), in vitro assays (colony-forming cells, microsphere, side-population, surface antigen staining, aldehyde dehydrogenase activity, and Paul Karl Horan la-bel-retaining cells, surface markers, nonenriched and enriched detection), reporter systems, and other analytical methods (flow cytometry, fluorescence microscopy/spectroscopy, etc.). The detailed information on methods to detect CSC, CTC, and EPC in this review will assist investigators in successful prognosis, diagnosis, and cancer treatment with greater ease.

Original language	English
Pages (from-to)	1-29
Number of pages	29
Journal	Journal of Environmental Pathology, Toxicology and Oncology
Volume	42
Issue number	3
DOIs	https://doi.org/10.1615/JEnvironPatholToxicolOncol.2022044456
State	Published - 2023
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

cancer stem cells
circulating tumor cells
detection
diagnosis
endothelial progenitor cells

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10.1615/JEnvironPatholToxicolOncol.2022044456

Cite this

Fuloria, S., Subramaniyan, V., Gupta, G., Sekar, M., Meenakshi, D. U., Sathasivam, K., Sudhakar, K., Alharbi, K. S., Almutairi, S. S., Almalki, W. H., & Fuloria, N. K. (2023). Detection of Circulating Tumor Cells and Epithelial Progenitor Cells: A Comprehensive Study. Journal of Environmental Pathology, Toxicology and Oncology, 42(3), 1-29. https://doi.org/10.1615/JEnvironPatholToxicolOncol.2022044456

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title = "Detection of Circulating Tumor Cells and Epithelial Progenitor Cells: A Comprehensive Study",

abstract = "Technological advancement to enhance tumor cells (TC) has allowed discovery of various cellular bio-markers: cancer stem cells (CSC), circulating tumor cells (CTC), and endothelial progenitor cells (EPC). These are re-sponsible for resistance, metastasis, and premetastatic conditions of cancer. Detection of CSC, CTC, and EPC assists in early diagnosis, recurrence prediction, and treatment efficacy. This review describes various methods to detect TC subpop-ulations such as in vivo assays (sphere-forming, serial dilution, and serial transplantation), in vitro assays (colony-forming cells, microsphere, side-population, surface antigen staining, aldehyde dehydrogenase activity, and Paul Karl Horan la-bel-retaining cells, surface markers, nonenriched and enriched detection), reporter systems, and other analytical methods (flow cytometry, fluorescence microscopy/spectroscopy, etc.). The detailed information on methods to detect CSC, CTC, and EPC in this review will assist investigators in successful prognosis, diagnosis, and cancer treatment with greater ease.",

keywords = "cancer stem cells, circulating tumor cells, detection, diagnosis, endothelial progenitor cells",

author = "Shivkanya Fuloria and Vetriselvan Subramaniyan and Gaurav Gupta and Mahendran Sekar and Meenakshi, \{Dhanalekshmi Unnikrishnan\} and Kathiresan Sathasivam and Kalvatala Sudhakar and Alharbi, \{Khalid Saad\} and Almutairi, \{Sultan Saadi\} and Almalki, \{Waleed Hassan\} and Fuloria, \{Neeraj Kumar\}",

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year = "2023",

doi = "10.1615/JEnvironPatholToxicolOncol.2022044456",

language = "English",

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Fuloria, S, Subramaniyan, V, Gupta, G, Sekar, M, Meenakshi, DU, Sathasivam, K, Sudhakar, K, Alharbi, KS, Almutairi, SS, Almalki, WH & Fuloria, NK 2023, 'Detection of Circulating Tumor Cells and Epithelial Progenitor Cells: A Comprehensive Study', Journal of Environmental Pathology, Toxicology and Oncology, vol. 42, no. 3, pp. 1-29. https://doi.org/10.1615/JEnvironPatholToxicolOncol.2022044456

TY - JOUR

T1 - Detection of Circulating Tumor Cells and Epithelial Progenitor Cells

T2 - A Comprehensive Study

AU - Fuloria, Shivkanya

AU - Subramaniyan, Vetriselvan

AU - Gupta, Gaurav

AU - Sekar, Mahendran

AU - Meenakshi, Dhanalekshmi Unnikrishnan

AU - Sathasivam, Kathiresan

AU - Sudhakar, Kalvatala

AU - Alharbi, Khalid Saad

AU - Almutairi, Sultan Saadi

AU - Almalki, Waleed Hassan

AU - Fuloria, Neeraj Kumar

PY - 2023

Y1 - 2023

N2 - Technological advancement to enhance tumor cells (TC) has allowed discovery of various cellular bio-markers: cancer stem cells (CSC), circulating tumor cells (CTC), and endothelial progenitor cells (EPC). These are re-sponsible for resistance, metastasis, and premetastatic conditions of cancer. Detection of CSC, CTC, and EPC assists in early diagnosis, recurrence prediction, and treatment efficacy. This review describes various methods to detect TC subpop-ulations such as in vivo assays (sphere-forming, serial dilution, and serial transplantation), in vitro assays (colony-forming cells, microsphere, side-population, surface antigen staining, aldehyde dehydrogenase activity, and Paul Karl Horan la-bel-retaining cells, surface markers, nonenriched and enriched detection), reporter systems, and other analytical methods (flow cytometry, fluorescence microscopy/spectroscopy, etc.). The detailed information on methods to detect CSC, CTC, and EPC in this review will assist investigators in successful prognosis, diagnosis, and cancer treatment with greater ease.

AB - Technological advancement to enhance tumor cells (TC) has allowed discovery of various cellular bio-markers: cancer stem cells (CSC), circulating tumor cells (CTC), and endothelial progenitor cells (EPC). These are re-sponsible for resistance, metastasis, and premetastatic conditions of cancer. Detection of CSC, CTC, and EPC assists in early diagnosis, recurrence prediction, and treatment efficacy. This review describes various methods to detect TC subpop-ulations such as in vivo assays (sphere-forming, serial dilution, and serial transplantation), in vitro assays (colony-forming cells, microsphere, side-population, surface antigen staining, aldehyde dehydrogenase activity, and Paul Karl Horan la-bel-retaining cells, surface markers, nonenriched and enriched detection), reporter systems, and other analytical methods (flow cytometry, fluorescence microscopy/spectroscopy, etc.). The detailed information on methods to detect CSC, CTC, and EPC in this review will assist investigators in successful prognosis, diagnosis, and cancer treatment with greater ease.

KW - cancer stem cells

KW - circulating tumor cells

KW - detection

KW - diagnosis

KW - endothelial progenitor cells

UR - https://www.scopus.com/pages/publications/85151792959

U2 - 10.1615/JEnvironPatholToxicolOncol.2022044456

DO - 10.1615/JEnvironPatholToxicolOncol.2022044456

M3 - Article

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AN - SCOPUS:85151792959

SN - 0731-8898

VL - 42

SP - 1

EP - 29

JO - Journal of Environmental Pathology, Toxicology and Oncology

JF - Journal of Environmental Pathology, Toxicology and Oncology

IS - 3

ER -

Detection of Circulating Tumor Cells and Epithelial Progenitor Cells: A Comprehensive Study

Abstract

UN SDGs

Keywords

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