Dengue NS1 Detection Using Electrochemical Techniques on Digital Microfluidics Platform

The early diagnosis and successful treatment of dengue fever depend on detecting dengue virus non-structural protein 1 (NS1). This study investigates the integration of electrochemical detection with digital microfluidics (DMF) to quickly and accurately detect NS1. DMF platforms offer precise manipulation of small volumes of fluids, enabling automated and high-throughput analysis. DMF platforms offer precise manipulation of small volumes of fluids, enabling automated and high-throughput analysis. In our approach, carbon-based electrodes with NS1 antibodies were utilised to capture the NS1 antigen from the sample. This study reviews the droplet size of various DMF applications that met the size of the 10 µl NS1 antigen droplet in our preliminary results. We employed cyclic voltammetry (CV) to measure the current response and correlated it with the NS1 concentration. Our findings show that this method can detect the presence of Dengue NS1 antigen, which is much more sensitive than other immunoassays. Additionally, the integration of DMF with electrochemical detection has potential for point-of-care diagnostics. Future work will focus on the miniaturisation and field deployment of the device, aiming to provide accessible diagnostics in resource-limited settings.