Controlling of Wound Pathogens using Iron Oxide Nanoparticles Mediated Through Hydrocotyle Umbellata L.-An Invitro Study

Novel nanomaterials have been discovered as a result of recent changes in nanoscience and nanotechnology, presenting the possibility of health and ecological hazards. Metallic nanoparticles that are affordable, energy-efficient, nontoxic, and biodegradable have been produced using biological resources like bacteria, fungi, algae, and plants. The purpose of this research is to learn about the antibacterial activity of Hydrocotyle umbellata mediated iron oxide nanoparticles and to see the antimicrobial activity against uropathogens. The time kill curve analysis was done to check the bactericidal properties of the nanoparticle against the uropathogens, Escherichia coli, Enterococcus faecalis, P. aeruginosa, and Streptococcus mutans. Preparation of the plant solution was done by using the plant powder. Followed by the preparation of the iron oxide nanoparticle solution. Antimicrobial activity and Time kill curve analysis against wound pathogens was done to check the properties of the iron oxide nanoparticle which was synthesized by the plant Hydrocotyle umbellata. The results have been graphically represented and it proves that the iron oxide nanoparticles are bactericidal, not only preventing the further growth of bacterias but also killing the remnants of the bacterias. This study concludes that iron oxide nanoparticles that were synthesized using the plant Hydrocotyle umbellata have an amazing antimicrobial property against wound pathogens, hence proving that it can be further modified for targeted drug therapy.