Comparative evaluation and efficacy of linezolid, vancomycin and ciprofloxacin on enterococcal induced biofilm using scanning electron microscopy an in vitro study

To evaluate and compare the effect and efficacy of linezolid, vancomycin and ciprofloxacin on enterococcal induced biofilm using scanning electron microscopy. Fifty mandibular premolars were selected for use in this study. Access cavity preparation was done. Working length was determined using periapical radiographs. Coronal third of the canals were flared using Gates Glidden drills. The canals were prepared up to size # 25 K file, 1mm short of apical foramen. Step back preparation was completed up to size # 50 K file. During instrumentation of the canals, canals were irrigated using sodium hypochlorite 5.25% and 17% EDTA. The teeth were then air-dried and steam autoclaved at 121°C for 30 minutes. Two longitudinal grooves were made along the entire length on opposite sides of the outer surface of the teeth to act as a guide for the subsequent splitting of the tooth into two halves. All the samples were divided into 5 groups (n=10). GROUP 1: Negative control. GROUP 2:Positive control. GROUP 3: Linezolid. GROUP 4: Vancomycin. GROUP 5: Ciprofloxacin. Residual aliquots were removed and spread on brain heart infusion broth and send for SEM to determine the percentage of negative culture obtained. Results were analysed using one-way ANOVA test and Duncan's test. A p-value less than 0.05was considered significant. The antimicrobial efficacy of linezolid was found to be higher than that of ciprofloxacin and vancomycin.