Characterization of a 66 kDa Secretory Protein of Haemonchus contortus and Its Effect on Host Mononuclear Cells

Murugavel Sokkalingam; Amir Kumar Samal; Lakshmi Prasanth Thangavelu; Paritosh Joshi

doi:10.1007/s40011-019-01162-1

Characterization of a 66 kDa Secretory Protein of Haemonchus contortus and Its Effect on Host Mononuclear Cells

Murugavel Sokkalingam
, Amir Kumar Samal
, Lakshmi Prasanth Thangavelu
, Paritosh Joshi

Indian Veterinary Research Institute

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Excretory/secretory (ES) products play an important role in the survival of Haemonchus contortus. Previously, a 66 kDa ES protein (P66) was identified that reacted with the infected animal sera and inhibited monocyte functions in vitro. In this study, the identity of P66 was established as glutamate dehydrogenase by mass spectrometry followed by enzymatic assay. This facilitated the generation of recombinant P66. The native and recombinant forms of the enzyme stimulated the proliferation of caprine peripheral blood mononuclear cells in vitro and also induced the production of cytokines IL-4 and IFN-γ, analogous to the immune response (Th1 and Th2), observed during natural infection. However, P66 showed no complement-inhibiting activity. The importance of P66 in the host–parasite relationship is discussed.

Original language	English
Pages (from-to)	895-902
Number of pages	8
Journal	Proceedings of the National Academy of Sciences India Section B - Biological Sciences
Volume	90
Issue number	4
DOIs	https://doi.org/10.1007/s40011-019-01162-1
State	Published - 1 Oct 2020
Externally published	Yes

Keywords

Cytokines
Glutamate dehydrogenase
Haemonchus contortus
IL-4 and IFN-γ
Secretory protein

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10.1007/s40011-019-01162-1

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title = "Characterization of a 66 kDa Secretory Protein of Haemonchus contortus and Its Effect on Host Mononuclear Cells",

abstract = "Excretory/secretory (ES) products play an important role in the survival of Haemonchus contortus. Previously, a 66 kDa ES protein (P66) was identified that reacted with the infected animal sera and inhibited monocyte functions in vitro. In this study, the identity of P66 was established as glutamate dehydrogenase by mass spectrometry followed by enzymatic assay. This facilitated the generation of recombinant P66. The native and recombinant forms of the enzyme stimulated the proliferation of caprine peripheral blood mononuclear cells in vitro and also induced the production of cytokines IL-4 and IFN-γ, analogous to the immune response (Th1 and Th2), observed during natural infection. However, P66 showed no complement-inhibiting activity. The importance of P66 in the host–parasite relationship is discussed.",

keywords = "Cytokines, Glutamate dehydrogenase, Haemonchus contortus, IL-4 and IFN-γ, Secretory protein",

author = "Murugavel Sokkalingam and Samal, \{Amir Kumar\} and Thangavelu, \{Lakshmi Prasanth\} and Paritosh Joshi",

note = "Publisher Copyright: {\textcopyright} 2020, The National Academy of Sciences, India.",

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doi = "10.1007/s40011-019-01162-1",

language = "English",

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Characterization of a 66 kDa Secretory Protein of Haemonchus contortus and Its Effect on Host Mononuclear Cells. / Sokkalingam, Murugavel; Samal, Amir Kumar; Thangavelu, Lakshmi Prasanth et al.
In: Proceedings of the National Academy of Sciences India Section B - Biological Sciences, Vol. 90, No. 4, 01.10.2020, p. 895-902.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterization of a 66 kDa Secretory Protein of Haemonchus contortus and Its Effect on Host Mononuclear Cells

AU - Sokkalingam, Murugavel

AU - Samal, Amir Kumar

AU - Thangavelu, Lakshmi Prasanth

AU - Joshi, Paritosh

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Excretory/secretory (ES) products play an important role in the survival of Haemonchus contortus. Previously, a 66 kDa ES protein (P66) was identified that reacted with the infected animal sera and inhibited monocyte functions in vitro. In this study, the identity of P66 was established as glutamate dehydrogenase by mass spectrometry followed by enzymatic assay. This facilitated the generation of recombinant P66. The native and recombinant forms of the enzyme stimulated the proliferation of caprine peripheral blood mononuclear cells in vitro and also induced the production of cytokines IL-4 and IFN-γ, analogous to the immune response (Th1 and Th2), observed during natural infection. However, P66 showed no complement-inhibiting activity. The importance of P66 in the host–parasite relationship is discussed.

AB - Excretory/secretory (ES) products play an important role in the survival of Haemonchus contortus. Previously, a 66 kDa ES protein (P66) was identified that reacted with the infected animal sera and inhibited monocyte functions in vitro. In this study, the identity of P66 was established as glutamate dehydrogenase by mass spectrometry followed by enzymatic assay. This facilitated the generation of recombinant P66. The native and recombinant forms of the enzyme stimulated the proliferation of caprine peripheral blood mononuclear cells in vitro and also induced the production of cytokines IL-4 and IFN-γ, analogous to the immune response (Th1 and Th2), observed during natural infection. However, P66 showed no complement-inhibiting activity. The importance of P66 in the host–parasite relationship is discussed.

KW - Cytokines

KW - Glutamate dehydrogenase

KW - Haemonchus contortus

KW - IL-4 and IFN-γ

KW - Secretory protein

UR - https://www.scopus.com/pages/publications/85077535690

U2 - 10.1007/s40011-019-01162-1

DO - 10.1007/s40011-019-01162-1

M3 - Article

AN - SCOPUS:85077535690

SN - 0369-8211

VL - 90

SP - 895

EP - 902

JO - Proceedings of the National Academy of Sciences India Section B - Biological Sciences

JF - Proceedings of the National Academy of Sciences India Section B - Biological Sciences

IS - 4

ER -

Characterization of a 66 kDa Secretory Protein of Haemonchus contortus and Its Effect on Host Mononuclear Cells

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Keywords

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