TY - GEN
T1 - Behavior of osteoclast cells response on dicalcium phosphate dihydrate layer-coated β-tricalcium phosphate granular
AU - Shariff, Khairul Anuar
AU - Abu Bakar, Mohamad Hafizi
AU - Cahyanto, Arief
N1 - Publisher Copyright:
© 2020 Trans Tech Publications Ltd, Switzerland.
PY - 2020
Y1 - 2020
N2 - The aim of this study is to investigate the behavior of osteoclast cells response on dicalcium phosphate dihydrate (DCPD) layer-coated β-Tricalcium Phosphate (β-TCP) granules. β-TCP granules with 300-600 µm were exposed to acidic calcium phosphate solution for 30 mins in order to get 10 mol% DCPD layer-coated β-TCP granular. DCPD free-coated β-TCP granular had used as control specimen. Both specimens were implant in 9 mm of rat calvarial bone defect for 4 weeks. After 4 weeks, the block section of rat calvarial containing specimen were removed for Tatrate-Resistance Acid Phosphatase (TRAP) analysis. Results of TRAP staining reveal that the number of osteoclast cells attached on 10 mol% layer-coated β-TCP granular is higher than DCPD free-coated β-TCP granular. Since remodeling of new bone formation involved simultaneous osteoclast and osteoblast cells response, therefore, the results obtained in this study indicated that the presence of DCPD layer-coated on β-TCP granular helps to improve osteoclast cells response that contribute in stimulating new bone formation.
AB - The aim of this study is to investigate the behavior of osteoclast cells response on dicalcium phosphate dihydrate (DCPD) layer-coated β-Tricalcium Phosphate (β-TCP) granules. β-TCP granules with 300-600 µm were exposed to acidic calcium phosphate solution for 30 mins in order to get 10 mol% DCPD layer-coated β-TCP granular. DCPD free-coated β-TCP granular had used as control specimen. Both specimens were implant in 9 mm of rat calvarial bone defect for 4 weeks. After 4 weeks, the block section of rat calvarial containing specimen were removed for Tatrate-Resistance Acid Phosphatase (TRAP) analysis. Results of TRAP staining reveal that the number of osteoclast cells attached on 10 mol% layer-coated β-TCP granular is higher than DCPD free-coated β-TCP granular. Since remodeling of new bone formation involved simultaneous osteoclast and osteoblast cells response, therefore, the results obtained in this study indicated that the presence of DCPD layer-coated on β-TCP granular helps to improve osteoclast cells response that contribute in stimulating new bone formation.
KW - Bioceramic
KW - Bone remodeling
KW - Calcium phosphate
KW - Ceramic coating
KW - Osteoclast cell
UR - https://www.scopus.com/pages/publications/85091715023
U2 - 10.4028/www.scientific.net/MSF.1010.549
DO - 10.4028/www.scientific.net/MSF.1010.549
M3 - Conference contribution
AN - SCOPUS:85091715023
SN - 9783035715828
T3 - Materials Science Forum
SP - 549
EP - 554
BT - Development and Investigation of Materials Using Modern Techniques II
A2 - Mohd Zaki, Ruhiyuddin
A2 - Che Pa, Faizul
A2 - Mohd Zaki, Ruhiyuddin
A2 - Ahmad, Khairel Rafezi
A2 - Che Pa, Faizul
A2 - Wahid, Mohd Fitri Mohamad
A2 - Wan Ibrahim, Wan Mohd Arif
A2 - Darus, Murizam
A2 - Ahmad, Zainal Arifin
A2 - Ismail, Khairul Nizar
A2 - Idris, Mohd Sobri
A2 - Jamil, Noorina Hidayu
A2 - Kasmuin, Mohd Zaheruddin
A2 - Ibrahim, Abdul Haqi
PB - Trans Tech Publications Ltd
T2 - 3rd International Conference on Advanced Materials Characterization Techniques, AMCT 2019
Y2 - 23 July 2019 through 24 July 2019
ER -