Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways

Steve Harakeh; Rajaa Al-Raddadi; Turki Alamri; Soad Al-Jaouni; Mohammed Qari; Yousef Qari; Ajay Kumar; Hanaa M. Tashkandi; Mohammed Moulay; Alia M. Aldahlawi; Petr Slama; Shafiul Haque

doi:10.1016/j.biopha.2023.115236

Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways

Steve Harakeh
, Rajaa Al-Raddadi
, Turki Alamri
, Soad Al-Jaouni
, Mohammed Qari
, Yousef Qari
, Ajay Kumar
, Hanaa M. Tashkandi
, Mohammed Moulay
, Alia M. Aldahlawi
, Petr Slama
, Shafiul Haque

Center of Medical and Bio-allied Health Sciences Research (CMBHSR)

King Fahd Medical Research Center
King Abdulaziz University
Chandigarh University
Faculty of Sciences, King Abdulaziz University
Mendel University in Brno
Jazan University
Lebanese American University

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase − 3 and − 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.

Original language	English
Article number	115236
Journal	Biomedicine and Pharmacotherapy
Volume	165
DOIs	https://doi.org/10.1016/j.biopha.2023.115236
State	Published - Sep 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antiproliferative
Apoptosis
DNA nicking
HepG2
Mitochondrial membrane potential (MMP)
Reactive oxygen species (ROS)

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10.1016/j.biopha.2023.115236

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Harakeh, S., Al-Raddadi, R., Alamri, T., Al-Jaouni, S., Qari, M., Qari, Y., Kumar, A., Tashkandi, H. M., Moulay, M., Aldahlawi, A. M., Slama, P., & Haque, S. (2023). Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways. Biomedicine and Pharmacotherapy, 165, Article 115236. https://doi.org/10.1016/j.biopha.2023.115236

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title = "Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways",

abstract = "trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase − 3 and − 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.",

keywords = "Antiproliferative, Apoptosis, DNA nicking, HepG2, Mitochondrial membrane potential (MMP), Reactive oxygen species (ROS)",

author = "Steve Harakeh and Rajaa Al-Raddadi and Turki Alamri and Soad Al-Jaouni and Mohammed Qari and Yousef Qari and Ajay Kumar and Tashkandi, \{Hanaa M.\} and Mohammed Moulay and Aldahlawi, \{Alia M.\} and Petr Slama and Shafiul Haque",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors",

year = "2023",

month = sep,

doi = "10.1016/j.biopha.2023.115236",

language = "English",

volume = "165",

journal = "Biomedicine and Pharmacotherapy",

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Harakeh, S, Al-Raddadi, R, Alamri, T, Al-Jaouni, S, Qari, M, Qari, Y, Kumar, A, Tashkandi, HM, Moulay, M, Aldahlawi, AM, Slama, P & Haque, S 2023, 'Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways', Biomedicine and Pharmacotherapy, vol. 165, 115236. https://doi.org/10.1016/j.biopha.2023.115236

TY - JOUR

T1 - Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways

AU - Harakeh, Steve

AU - Al-Raddadi, Rajaa

AU - Alamri, Turki

AU - Al-Jaouni, Soad

AU - Qari, Mohammed

AU - Qari, Yousef

AU - Kumar, Ajay

AU - Tashkandi, Hanaa M.

AU - Moulay, Mohammed

AU - Aldahlawi, Alia M.

AU - Slama, Petr

AU - Haque, Shafiul

PY - 2023/9

Y1 - 2023/9

N2 - trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase − 3 and − 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.

AB - trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase − 3 and − 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.

KW - Antiproliferative

KW - Apoptosis

KW - DNA nicking

KW - HepG2

KW - Mitochondrial membrane potential (MMP)

KW - Reactive oxygen species (ROS)

UR - https://www.scopus.com/pages/publications/85166235565

U2 - 10.1016/j.biopha.2023.115236

DO - 10.1016/j.biopha.2023.115236

M3 - Article

C2 - 37531780

AN - SCOPUS:85166235565

SN - 0753-3322

VL - 165

JO - Biomedicine and Pharmacotherapy

JF - Biomedicine and Pharmacotherapy

M1 - 115236

ER -

Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways

Abstract

UN SDGs

Keywords

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