An Analysis of the Physicochemical Properties of Total Fill Bio Ceramic Root Canal Sealer Loaded with Chitosan Nanoparticles

This study aimed to determine the incorporation of Total Fill endodontic bioceramic sealer with the chitosan nanoparticles, where the relationship between the degree of conversion (DC) and antimicrobial effect were assessed. Total Fill bioceramic sealers mixed with chitosan nanoparticles in different concentrations (2.5%,5%,10%). The antibacterial activity against Enterococcus faecalis was measured by the direct contact test (DCT) at different time intervals (immediately, 30 min., 24 hours, 48 hours, 7 days). Fourier Transformed Infrared Spectroscopy measures the degree of conversion. The Kruskal−Wallis and Mann-Whitney U tests were used to analyze group differences and a post hoc comparison of Duncan’s multiple range test. Pearson correlation test and Linear regression analysis were used to determine the correlation between the degree of conversion and antibacterial activity between groups. The statistical analyses were computed at a 0.05 significance level. There is a significant difference between the readings of antibacterial activity and the degree of conversion at different time intervals (P=0.05). The antibacterial activity increased as the nanoparticle concentrations increased, and the degree of conversion decreased as the chitosan nanoparticles increased. Positive, strong relation between the degree of conversion and antimicrobial activity.